Journal: Vaccines

Article Title: Augmenting Vaccine Efficacy against Delta Variant with ‘Mycobacterium- w ’-Mediated Modulation of NK-ADCC and TLR-MYD88 Pathways

doi: 10.3390/vaccines11020328

Figure Lengend Snippet: Adaptive NK (ANK) cells and inhibitory NK (iNK) cells: Scatter dot with bar plot showing ( A ) the kinetics of NKG2C + ANK and NKG2A + iNK cells in Mw + ChAdOx1 group at baseline, day 30, day 60, day 100 and day 180. ( B ) Expression of NKG2C + ANK and NKG2A + iNK cells between Mw + ChAdOx1 and ChAdOx1 group at the time of enrolment. Red shaded triangles and dots represent NKG2C + ANK and NKG2A + iNK, respectively, for Mw + ChAdOx1 group. Black shaded triangles and dots represent NKG2C + ANK and NKG2A + iNK, respectively, for ChAdOx1 group. ** p < 0.01, *** p < 0.001, **** p < 0.0001 and ns = p -value not significant.

Article Snippet: For surface staining, 0.5 × 10 6 cells were washed with phosphate-buffered saline (PBS) and stained with the following antibodies, which were used for phenotypic analysis: CD3 (APC-H7, SK-7) CD16 (PE-Cy7, B73.1), CD56 (APC R700, NCAM16.2) and NKG2A (PE-Cy7, Z199), from BD Biosciences, (San Jose, CA, USA) and NKG2C (PE, REA205) from Miltenyi Biotec, Bergisch Gladbach, North Rhine-Westphalia, Germany.

Techniques: Expressing